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By  Andrea Wild 4 March 2024 4 min read

Key points

  • A fossil site in New South Wales has preserved extraordinary details of more than 100 immature caddisflies.
  • The site has preserved details of their compound eyes, including their nanocoating.
  • The fossils show amazing details of their silk glands, wings, claws and more, providing new information about the past.

Around 11 to 16 million years ago, in the middle of the Miocene period, more than 100 caddisflies met their end in a lake.

Over time, the iron-rich sediments at the bottom of the lake formed a thick layer of the mineral goethite. Along with many other animals, the caddisflies became fossilised in a Konservat-Lagerstätte, a name given to a fossil site that preserves extremely fine details. The site is named McGraths Flat and is located in central New South Wales.

The caddisflies are so well preserved that the nanostructure of their eyes could inspire new products. These could range from antimicrobial paints to sunglasses that repel water.

Photograph of a pharate caddisfly from the McGraths Flat fossil site. Pharate insects are fully developed adults that have not yet left the pupal cuticle and expanded their wings. Image: Michael Frese

Fossilised fossil eyes

Caddisflies are small, moth-like insects with hairy wings. They are an order of insects known to scientists as Trichoptera. Fly fishers call them sedges.

Caddisflies spend most of their lives as aquatic larvae. They also pupate underwater.

The caddisflies preserved at McGraths Flat died as larvae and pharate adults. Pharate adults are pupae that have completed metamorphosis but have not yet broken free of their pupal cuticle. This cling-wrap like covering protects them until they fly free from the water’s surface.

Dr Michael Frese is a visiting scientist who worked with Dr Alice Wells at our Australian National Insect Collection and Matthew McCurry from the Australian Museum. Together, they studied the caddisflies at McGraths Flat.

“Using a scanning electron microscope, we could see details of the caddisflies’ compound eyes, silk glands, mouthparts, gastrointestinal tract, claws and genitalia. We even found pollen inside the gut of one caddisfly,” Michael said.

“Most remarkable were two fossils that showed the corneal nanocoating. This is the molecular structure on the surface of the caddisflies’ compound eyes.

“We think these might be among the best-preserved fossilised eyes on the planet.”

Scanning electron microscope (SEM) image showing the highly regular hexagonal pattern of the corneal nanocoating on the eyes of a pharate caddisfly. Image: Michael Frese

Michael said the nanocoating suggests the eyes were anti-reflective, perhaps because the caddisflies were adapted to low light.

“The surface was super hydrophobic, both chemically because it was a mixture of protein and wax, and also structurally. Its pattern might inspire new coatings for glass windscreens or sunglasses,” he said.

"The surface is also anti-bacterial because it stresses the outer membrane of Gram-negative bacteria. It could inspire paint to make hospital walls resistant to bacterial growth.”

Michael said that he has seen a similar coating on the wings of a fossil cicada from the same site. The cicadas from the site are the only fossil cicadas from Australia. They are also by far the biggest cicada fossils on the planet.

Revealing the secrets of ancient insects

The fossil caddisflies of the McGraths Flat fossil site reveal how well the mineral goethite can preserve soft tissues.

“The only time you might see a better-preserved insect is in amber. Yet, because amber forms from the sticky sap of trees, it tends to only preserve adult insects, not their aquatic larvae,” Michael said.

Insects in amber are also very difficult to prepare for a scanning electron microscope. You have to slice the amber into thin sections, destroying the fossil. In contrast, the team could examine the fossils from McGraths Flat without damaging them. 

Michael said one amazing fossil showed both the claws of a caddisfly and the pupal cuticle that had protected its claws.

“It is like removing your hand from a glove, and the glove still holding the shape of your fingers.”

Scanning electron microscope (SEM) image showing the claws on the leg of a pharate caddisfly. Before fossilisation, the leg has been partially pulled out of the pupal cuticle. Red arrows indicate the position of the claws. White and yellow arrows indicate the position of the cuticle that fitted the leg like a 'glove'.

Using fossils to reconstruct the past

Alice and Michael considered whether they could describe the two distinct kinds of caddisflies at McGraths Flat as new species. They would need to see the pattern of the wing venation (the pattern of veins in the wings) and the external reproductive parts of males. 

Unfortunately, this was not possible.

“Only pharate adults were found so far,” Alice said.

“They were trapped in the sediment before breaking from their pupal cuticles and expanding their wings to fly away. As a result, their wing veins are not visible,” she said.

But the fossils did reveal other details about the past.

This fossil shows the well-developed silk glands of a caddisfly larva. Image: Michael Frese

“We see larvae without cases and pupae without cocoons. This suggests one or more events drove the larvae from their silken retreats and the pupae from their cocoons,” Alice said.

According to Matthew, the findings suggest According to Matthew, the findings suggest an unstable paleoenvironment (the environment at that time in the past).

“This contributed to the preservation of a very wide range of Miocene plants and animals at McGraths Flat,” he said.

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